AFB Culture Reflex to TBWGS - Pulmonary

The AFB Culture Reflex to TBWGS – Pulmonary test is a specialized laboratory test used to detect acid-fast bacilli (AFB) from pulmonary specimens such as sputum or bronchial samples. If Mycobacterium tuberculosis is isolated during culture, the sample is automatically referred for Tuberculosis Whole Genome Sequencing (TBWGS). This advanced genetic analysis provides detailed information about the bacterial strain and helps identify mutations associated with drug resistance. The test supports accurate diagnosis and offers valuable insights for planning appropriate tuberculosis treatment.

Who should get this test done?

This test may be recommended for individuals who:

●     Have symptoms suggestive of pulmonary tuberculosis, such as a persistent cough, fever, or unexplained weight loss.

●     Require culture-based confirmation of suspected pulmonary TB.

●     Have a positive screening test for tuberculosis and need further microbiological evaluation.

●     Are suspected of having drug-resistant tuberculosis.

●     Need comprehensive laboratory assessment of a pulmonary mycobacterial infection as advised by their healthcare provider.

Why consider this test?

Pulmonary tuberculosis requires accurate identification of the causative organism to ensure appropriate treatment. This test combines the reliability of AFB culture with the added benefit of Whole Genome Sequencing (TBWGS) whenever Mycobacterium tuberculosis is detected. The sequencing provides detailed genetic information, including mutations associated with resistance to anti-tubercular medications, helping clinicians select the most suitable treatment strategy while supporting effective disease management.

More information

The test begins with culturing a pulmonary specimen to detect the growth of Mycobacterium tuberculosis or other acid-fast bacilli. If M. tuberculosis complex is identified, the isolate is automatically processed for TB Whole Genome Sequencing (TBWGS) without requiring an additional test request. The sequencing data assists in identifying the bacterial strain and evaluating genetic markers linked to drug resistance. This integrated approach provides a comprehensive laboratory assessment and is interpreted alongside clinical findings, imaging studies, and other microbiological investigations.

Other names

●     Pulmonary TB culture with genome sequencing
●     AFB culture with TB-WGS, pulmonary
●     TB WGS reflex test
●     Mycobacterium tuberculosis drug resistance genome panel
●     Sputum AFB culture with reflex sequencing