GCV Resistance by Sequencing

What is the GCV Resistance by Sequencing test?

This test detects resistance mutations to ganciclovir (GCV), an antiviral drug, by analyzing the UL97 and UL54 genes of cytomegalovirus (CMV). It helps assess whether a patient infected with CMV has developed resistance to GCV treatment.


Why is this test done?

It is primarily performed in immunocompromised individuals, such as transplant recipients or those with HIV/AIDS, who are undergoing CMV therapy. The test determines whether the virus has developed mutations rendering GCV ineffective.


How is the test performed?

A blood sample is collected, and viral DNA is extracted and sequenced to look for known mutations associated with resistance to ganciclovir.


What do the results mean?

Detection of resistance mutations implies that ganciclovir may no longer be effective, and alternative antiviral therapy should be considered. If no resistance is found, GCV treatment can be continued.


Are there any precautions or preparations required?

No special preparation is required. A standard blood draw is sufficient for analysis.